ASSESSMENT OF INDOOR SECOND-HAND CIGARETTE SMOKE ON THE RESPIRATORY MECHANICS AND PREVENTIVE BENEFITS OF EUCALYPTOL: A MURINE MODEL
DOI:
https://doi.org/10.32435/envsmoke-2023-0018Palavras-chave:
Second-hand smoke, Indoor pollution, Tobacco Smoke Pollution, Respiratory mechanics, EucalyptolResumo
Second-hand smokers, in various settings such as recreational areas, schools, workplaces, homes and other enclosed public spaces also experience the detrimental effects of exposure to cigarette smoke (CS). Out of the 8 million deaths attributed to smoking, 1.2 million are a result of non-smokers being exposed to second-hand smoke. As a preventive strategy to these damages, Eucalyptol has been shown to be effective in the treatment of lung lesions caused by smoking. This study aimed to assess the effects of indoor second-hand cigarette smoke on respiratory mechanics and lung tissue, in addition to investigating the potential benefit of Eucalyptol against lung damage caused by this type of aggression. The experiments were conducted for 14 days with 40 BALB/c mice, divided into 5 groups: a control group, two groups nebulized with saline solution, divided into active and passive (second-hand) exposure to CS, two groups pre-treated with eucalyptol (10 mg/mL by nebulization) divided into active and passive exposure to CS. Lung mechanics data were collected on a flexiVent® small animal mechanical ventilator. In addition, ex vivo analysis of lung tissue micromechanics, morphometric analyzes and qualitative analyzes of lung tissue were performed. The results showed that 14 days of second-hand exposure generated significant tissue damage, causing infiltration of inflammatory cells and altering respiratory mechanics. However, pre-treatment with eucalyptol was able to prevent all changes caused by CS in both exposure protocols. We conclude that indoor second-hand smoke is capable of causing damage similar to active tobacco consumption and that Eucalyptol can be used as a preventive or nutritional resource against the changes caused by the inhalation of cigarette smoke.
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ADAM, T.; MCAUGHEY, J.; MOCKER, C.; MCGRATH, C.; ZIMMERMANN, R. Influence of filter ventilation on the chemical composition of cigarette mainstream smoke. Analytica Chimica Acta, v. 657, n. 1, p. 36–44, 2010. Available from: https://doi.org/10.1016/j.aca.2009.10.015. DOI: https://doi.org/10.1016/j.aca.2009.10.015
AMARAL, E.P.; ROSA, R.C.; ETCHEBEHERE, R.M.; NOGUEIRA, R.D.; VOLPON, J.B.; RODRIGUES, D.B.R.; PEREIRA, S.A.L. Overexpression of HIF-1α and Morphological Alterations in the Tongue of Rats Exposed To Secondhand Smoke. Brazilian Dental Journal, v. 31, n. 3, p. 281–289,2020. Available from: https://doi.org/10.1590/0103-6440202002898. DOI: https://doi.org/10.1590/0103-6440202002898
AMERE, G.A.; NAYAK, P.; SALINDRI, A.D.; NARAYAN, K.M.V.; MAGEE, M.J. Contribution of Smoking to Tuberculosis Incidence and Mortality in High-Tuberculosis-Burden Countries. American journal of epidemiology, v. 187, n. 9, p.1846–1855, 2018. Available from: https://doi.org/10.1093/aje/kwy081. DOI: https://doi.org/10.1093/aje/kwy081
BALTAR, J.A.; SANTOS, M.S.B.; SILVA, H.J.A.; FILHO, N.T.P; Does asthma promote changes in static posture? - Systematic review. Revista Portuguesa de Pneumologia, v. 16, n. 3, p. 471-476, 2010. Available from: https://doi.org/10.1016/S2173-5115(10)70051-8. DOI: https://doi.org/10.1016/S2173-5115(10)70051-8
BARNES, P.J. New anti-inflammatory targets for chronic obstructive pulmonary disease. Nature reviews Drug discovery, v. 12, n. 7, p. 543-559, 2013. Available from: https://doi.org/10.1038/nrd4025. DOI: https://doi.org/10.1038/nrd4025
BATES, J.T. Lung mechanics: An inverse modeling approach. Cambridge: Cambridge University Press. 2009. Available from: https://doi.org/10.1017/CBO9780511627156. DOI: https://doi.org/10.1017/CBO9780511627156
BROWNE, C.L.; KEITH, C.H.; ALLEN, R.E. The effect of filter ventilation on the yield and composition of mainstream and sidestream smokes. Beiträge zur Tabakforschung International [Contributions to Tobacco & Nicotine Research], v. 10, n. 2, p. 81-90, 1980. Available from: https://sciendo.com/article/10.2478/cttr-2013-0473. Accessed on: 25 May 2023. DOI: https://doi.org/10.2478/cttr-2013-0473
CANTIN, A.M; RICHTER, M.V. Cigarette smoke-induced proteostasis imbalance in obstructive lung diseases. Current Molecular Medicine, v. 12, n. 7, p. 836-849, 2012. Available from: https://doi.org/10.2174/156652412801318746. DOI: https://doi.org/10.2174/156652412801318746
CARRERAS, G., LUGO, A., GALLUS, S., CORTINI, B., FERNÁNDEZ, E., LÓPEZ, M. J., SORIANO, J. B., LÓPEZ-NICOLÁS, A., SEMPLE, S., GORINI, G. Burden of disease attributable to second-hand smoke exposure: A systematic review. Preventive medicine, v. 129, p. 105833, 2019. Available from: https://doi.org/10.1016/j.ypmed.2019.105833. DOI: https://doi.org/10.1016/j.ypmed.2019.105833
CASTARDELI, É.; PAIVA, S.A.R.; MATSUBARA, B.B.; MATSUBARA, L.S.; MINICUCCI, M. F.; AZEVEDO, P. S.; CAMPANA, Á.O.; ZORNOFF, L.A.M. Chronic exposure to cigarette smoke leads to cardiac remodeling and impairment of ventricular function in rats. Brazilian Archives of Cardiology, v. 84, n. 4, p. 320–324, 2005. Available from: https://doi.org/10.1590/S0066-782X2005000400009. DOI: https://doi.org/10.1590/S0066-782X2005000400009
CAVALCANTE, F.S.Á.; ITO, S.; BREWER, K.; SAKAI, H.; ALENCAR, A.M.; ALMEIDA, M.P.; ANDRADE, J.S.J.; MAJUMDAR, A.; INGENITO, E.P.; SUKI, B. Mechanical interactions between collagen and proteoglycans: implications for the stability of lung tissue, Journal of Applied Physiology, v. 98, n. 2, p. 672- 679, 2005. Available from: http://doi.org/10.1152/japplphysiol.00619.2004. DOI: https://doi.org/10.1152/japplphysiol.00619.2004
CLAIRE S.S.; GOUDA, H.; SCHOTTE, K.; FAYOKUN, R.; FU, D.; VARGHESE, C.; PRASAD, V.M. Lung health, tobacco, and related products: gaps, challenges, new threats, and suggested research. American journal of physiology - Lung cellular and molecular physiology, v. 318, n. 5, p. L1004–L1007, 2020. Available from: https://doi.org/10.1152/ajplung.00101.2020. DOI: https://doi.org/10.1152/ajplung.00101.2020
CHEN, M.; HU, Q.; WANG, S.; TAO, L.; HU, X.; SHEN, X. 1,8-Cineole ameliorates endothelial injury and hypertension induced by L-NAME through regulation of autophagy via PI3K/mTOR signaling pathway. European Journal of Pharmacology, v. 954, 2023. Available from: https://doi.org/10.1016/j.ejphar.2023.175863. DOI: https://doi.org/10.1016/j.ejphar.2023.175863
COMPTON, C.; MCBRYAN, D.; BUCCHIONI, E.; PATALANO, F. The Novartis view on emerging drugs and novel targets for the treatment of chronic obstructive pulmonary disease. Pulmonary pharmacology & therapeutics, v. 26, n. 5, p. 562–573,2013. Available from: https://doi.org/10.1016/j.pupt.2013.05.009. DOI: https://doi.org/10.1016/j.pupt.2013.05.009
GONDIM, F.L.; SERRA, D.S.; CAVALCANTE, F.S.Á. Effects of Eucalyptol in respiratory system mechanics on acute lung injury after exposure to short-term cigarette smoke. Respiratory physiology & neurobiology, v. 266, p. 33–38, 2019. Available from: https://doi.org/10.1016/j.resp.2019.04.007. DOI: https://doi.org/10.1016/j.resp.2019.04.007
GRASSO, S.; STRIPOLI, T.; De MICHELE, M.; BRUNO, F.; MOSCHETTA, M.; ANGELELLI, G.; MUNNO, I.; RUGGIERO, V.; ANACLERIO, R.; CAFARELLI, A.; DRIESSEN, B.; FIORE, T. ARDSnet ventilatory protocol and alveolar hyperinflation: role of positive end-expiratory pressure. American journal of respiratory and critical care medicine, v. 176, n. 8, p. 761–767, 2007. Available from: https://doi.org/10.1164/rccm.200702-193OC. DOI: https://doi.org/10.1164/rccm.200702-193OC
HIZUME, C.D. Effects of exposure to cigarette smoke on inflammation, responsiveness, and lung remodeling in mice with chronic allergic lung inflammation. 2010. 105p. Thesis (Ph.D. in Sciences) — Federal University of São Paulo, São Paulo, 2010. Available from: https://doi.org/10.11606/T.5.2010.tde-26082010-172623. DOI: https://doi.org/10.11606/T.5.2010.tde-26082010-172623
IWASAKI, S.; YAMAKAGE, M.; SATOH, J.; NAMIKI A. Different inhibitory effects of sevoflurane on hyperreactive airway smooth muscle contractility in ovalbumin-sensitized and chronic cigarette-smoking guinea pig models. Anesthesiology, v. 105, n. 4, p. 753-763, 2006. Available from: https://doi.org/10.1097/00000542-200610000-00022. DOI: https://doi.org/10.1097/00000542-200610000-00022
JUERGENS, U.R.; ENGELEN, T.; RACKÉ, K.; STÖBER, M.; GILLISSEN, A.; VETTER, H. Inhibitory activity of 1,8-cineol (eucalyptol) on cytokine production in cultured human lymphocytes and monocytes. Pulmonary pharmacology & therapeutics, v. 17, n. 5, p. 281–287, 2004. Available from: https://doi.org/10.1016/j.pupt.2004.06.002. DOI: https://doi.org/10.1016/j.pupt.2004.06.002
JUERGENS, U.R. Anti-inflammatory properties of the monoterpene 1.8-cineole: current evidence for co-medication in inflammatory airway diseases. Drug research, v. 64 n. 12, p. 638–646, 2014. Available from: https://doi.org/10.1055/s-0034-1372609. DOI: https://doi.org/10.1055/s-0034-1372609
KENNEDY-FEITOSA, E.; CATTANI-CAVALIERI, I.; BARROSO, M. V.; ROMANA-SOUZA, B.; BRITO-GITIRANA, L.; VALENCA, S. S. Eucalyptol promotes lung repair in mice following cigarette smoke-induced emphysema. Phytomedicine: International Journal of Phytotherapy and Phytopharmacology, v. 55, p. 70–79, 2019. Available from: https://doi.org/10.1016/j.phymed.2018.08.012. DOI: https://doi.org/10.1016/j.phymed.2018.08.012
LAURELL, C.B.; ERIKSSON, S. The Electrophoretic α1-Globulin Pattern of Serum in α1-Antitrypsin Deficiency. COPD: Journal of Chronic Obstructive Pulmonary Disease, v. 10, n. 1, p. 3-8, 2013. Available from: http://dx.doi.org/10.3109/15412555.2013.771956. DOI: https://doi.org/10.3109/15412555.2013.771956
LEE, H.S.; PARK, D.E.; SONG, W.J.; PARK, H.W.; KANG, H.R.; CHO, S.H.; SOHN, S.W. Effect of 1.8-Cineole in Dermatophagoides pteronyssinus-Stimulated Bronchial Epithelial Cells and Mouse Model of Asthma. Biological and Pharmaceutical Bulletin, v. 39, n. 6, p. 946–952, 2016. Available from: https://doi.org/10.1248/bpb.b15-00876. DOI: https://doi.org/10.1248/bpb.b15-00876
LI, Y.; LAI, Y.; WANG, Y.; LIU, N.; ZHANG, F.; XU, P. 1, 8-Cineol protect against influenza-virus-induced pneumonia in mice. Inflammation, v. 39, n. 4, p. 1582-1593. 2016. Available from: https://doi.org/10.1007/s10753-016-0394-3. DOI: https://doi.org/10.1007/s10753-016-0394-3
MAIA-FILHO, A.L.M.; SILVA, V.S.; BARROS, T.L.; COSTA, C.L.S.; MAIA, E.P.V.D.; ARAÚJO, K.S.; SANTOS, I.M.S.P.; VILLAVERDE, A.G.J.B.; CARVALHO, F.A.S.; CARVALHO, R.A. Effect of Aloe Vera gel (Aloe barbadensis Mill.) associated with ultrasound in acute inflammatory process. Brazilian Journal of Medicinal Plants, v. 13, n. 2, p. 146–150, 2011. Available from: https://doi.org/10.1590/S1516-05722011000200004. DOI: https://doi.org/10.1590/S1516-05722011000200004
OBERG, M.; JAAKKOLA, M.S.; WOODWARD, A.; PERUGA, A.; PRÜSS-USTÜN, A. Worldwide burden of disease from exposure to second-hand smoke: a retrospective analysis of data from 192 countries. Lancet, v. 377, n. 9760, p. 139–146, 2011. Available from: https://doi.org/10.1016/S0140-6736(10)61388-8. DOI: https://doi.org/10.1016/S0140-6736(10)61388-8
OLIVEIRA, R.C.; PIRES NETO, R.C.; BUSTILLOS, J.O.; MACCHIONE, M.; DOLHNIKOFF, M.; SALDIVA, P.H.; GARCIA, M.L. Chemical composition modulates the adverse effects of particles on the mucociliary epithelium. Clinics, v. 70, p. 706-7013, 2015. Available from: https://doi.org/10.6061/clinics/2015(10)09. DOI: https://doi.org/10.6061/clinics/2015(10)09
ROMERO, P.V.; ZIN, W.A.; LOPEZ-AGUILAR, J. Frequency characteristics of lung tissue strip during passive stretch and induced pneumoconstriction. Journal of Applied Physiology, v. 91, n. 2, p. 882–890, 2001. Available from: https://doi.org/10.1152/jappl.2001.91.2.882. DOI: https://doi.org/10.1152/jappl.2001.91.2.882
SADLON, A.E.; LAMSON, D.W. Immune-modifying and antimicrobial effects of Eucalyptus oil and simple inhalation devices. Alternative Medicine Review: a Journal of Clinical Therapeutic, v. 15, n. 1, p. 33-47, 2010. Available from: https://pubmed.ncbi.nlm.nih.gov/20359267/. Accessed on: 17 july 2023.
SALAZAR, E.; KNOWLES, J.H. An analysis of pressure-volume characteristics of the lungs. Journal of Applied Physiology, v. 19, p. 97-104, 1964. Available from: https://doi.org/10.1152/jappl.1964.19.1.97. DOI: https://doi.org/10.1152/jappl.1964.19.1.97
SCHROEDER, S.A.; WARNER, K.E. Don’t Forget Tobacco. The New England journal of medicine, v. 363, n. 3, p. 201-204, 2010. Available from: https://doi.org/10.1056/NEJMp1003883. DOI: https://doi.org/10.1056/NEJMp1003883
SELKE, W.A. Dilution of smoke through ventilation of filters. Beiträge zur Tabakforschung International, v. 9, n. 4, p. 190, 1978. Available from: https://doi.org/10.2478/cttr-2013-0446. DOI: https://doi.org/10.2478/cttr-2013-0446
SERRA, D.S.; BRITO, K.B.P.; OLIVEIRA, K.L.; OLIVEIRA, M.L.M.; CAVALCANTE, F.S.Á. Respiratory System of Rats Exposed to Pollutants arising out of Heating Residual Glycerol. Journal of Fundamentals of Renewable Energy and Applications, v. 7, n. 1, 2017. Available from: https://www.longdom.org/abstract/respiratory-system-of-rats-exposed-to-pollutants-arising-out-of-heating-residual-glycerol-51410.html. Accessed on: 11 May 2023. DOI: https://doi.org/10.4172/2090-4541.1000220
SERRA, D.S.; SOUSA, A.M.; ANDRADE, L.C.S.; GONDIM, F.L.; SANTOS, J.E.Á.; OLIVEIRA, M.L.M.; PIMENTA, A.T.Á. Effects of fixed oil of Caryocar coriaceum Wittm. seeds on the respiratory system of rats in a short-term secondhand-smoke exposure model. Journal of Ethnopharmacology, v. 252, p. 112633, 2020. Available from: https://doi.org/10.1016/j.jep.2020.112633. DOI: https://doi.org/10.1016/j.jep.2020.112633
SOCIEDADE BRASILEIRA DE PNEUMOLOGIA E TISIOLOGIA; SOCIEDADE BRASILEIRA DE CARDIOLOGIA; ASSOCIAÇÃO BRASILEIRA DE PSIQUIATRIA; FEDERAÇÃO BRASILEIRA DAS SOCIEDADES DE GINECOLOGIA E OBSTETRÍCIA; SOCIEDADE BRASILEIRA DE ANESTESIOLOGIA; ASSOCIAÇÃO BRASILEIRA DE MEDICINA INTENSIVA; SOCIEDADE BRASILEIRA DE CANCEROLOGIA; SOCIEDADE BRASILEIRA DE PEDIATRIA. Tabagismo: parte I. Revista da Associação Médica Brasileira, v. 56, n. 2, p. 134-137, 2010. Available from: https://doi.org/10.1590/S0104-42302010000200005. DOI: https://doi.org/10.1590/S0104-42302010000200005
STEIDL, E.M.S.; FRIEDRICH, F.F.; ANTUNES, V.P.; BEUTER, C.R. Characterization of body posture and breathing pattern in asthmatic children [Avaliação da postura corporal e do padrão respiratório em crianças asmáticas]. Saúde (Santa Maria), v. 39, n. 1, p. 131–138, 2013. Available from: https://doi.org/10.5902/223658346416. DOI: https://doi.org/10.5902/223658346416
TACAO, G.Y.; RAMOS, E.M.C.; PESTANA, P.R.S.; CARVALHO JUNIOR, L.C.S.; FREIRE, A.P.C.F.; RAMOS, D. Analysis of mucociliary clearance smokers in intervals of smoking abstinence [Análise do transporte mucociliar de tabagistas em intervalos de abstinência do tabaco]. Colloquium Vitae, v. 6, n. 2, p. 22–30, 2015. Available from: https://revistas.unoeste.br/index.php/cv/article/view/1192. DOI: https://doi.org/10.5747/cv.2014.v06.n2.v095
TAM, A.; WADSWORTH, S.; DORSCHEID, D.; MAN, S.F.; SIN, D. D. The airway epithelium: more than just a structural barrier. Therapeutic advances in respiratory disease, v. 5, n. 4, p. 255–273, 2011. Available from: https://doi.org/10.1177/1753465810396539. DOI: https://doi.org/10.1177/1753465810396539
TRINDADE, S.H.K.; MELLO JÚNIOR, J.F.; MION, O.G.; LORENZI-FILHO, G.; MACCHIONE, M.; GUIMARÃES, E.T.; SALDIVA, P.H.N. Methods for studying mucociliary transport [Métodos de estudo do transporte mucociliar]. Revista Brasileira de Otorrinolaringologia, v. 73, n. 5, p.704–712, 2007. Available from: https://doi.org/10.1590/S0034-72992007000500018. DOI: https://doi.org/10.1590/S0034-72992007000500018
VALENÇA, S.S; PORTO, L.C. Immunohistochemical study of lung remodeling in mice exposed to cigarette smoke [Estudo imunohistoquímico do remodelamento pulmonar em camundongos expostos à fumaça de cigarro]. Jornal Brasileiro de Pneumologia, v. 34, n. 10, p. 787–795, 2008. Available from: https://doi.org/10.1590/s1806-37132008001000006. DOI: https://doi.org/10.1590/S1806-37132008001000006
WONG, J.; MAGUN, B.E.; WOOD, L.J. Lung inflammation caused by inhaled toxicants: a review. International Journal of Chronic Obstructive Pulmonary Disease, v. 11, n. 1, p. 1391-1401, 2016. Available from: https://doi.org/10.2147/COPD.S106009. DOI: https://doi.org/10.2147/COPD.S106009
WORLD HEALTH ORGANIZATION. Tobacco Fact Sheet. Genebra: World Health Organization, 2020. Available from: https://www.who.int/docs/default-source/campaigns-and-initiatives/world-no-tobacco-day-2020/wntd-tobacco-fact-sheet.pdf. Accessed on: 04 May. 2022
WÜNSCH FILHO, V.; MIRRA, A.P.; LÓPEZ, R.V.M.; ANTUNES, L.F. Tobacco smoking and cancer in Brazil: evidence and prospects [Tabagismo e câncer no Brasil: evidências e perspectivas]. Revista Brasileira de Epidemiologia, v. 13, n. 2, p. 175–187. Available from: https://doi.org/10.1590/S1415-790X2010000200001. DOI: https://doi.org/10.1590/S1415-790X2010000200001
XU, G.; GUO, J.; SUN, C. Eucalyptol ameliorates early brain injury after subarachnoid haemorrhage via antioxidant and anti-inflammatory effects in a rat model. Pharmaceutical Biology, v. 59, n. 1, p. 112-118, 2021. Available from: https://doi.org/10.1080/13880209.2021.1876101. DOI: https://doi.org/10.1080/13880209.2021.1876101
YPSILANTIS, P.; POLITOU, M.; ANAGNOSTOPOULOS, C.; KORTSARIS, A.; SIMOPOULOS, C. A rat model of cigarette smoke abuse liability. Comparative Medicine, v. 62, n. 5, p. 395-399, 2012. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472604/. Accessed on: 17 May 2023.
ZHAO, C.; SUN, J.; FANG, C.; TANG, F. 1,8-Cineol attenuates LPS-induced acute pulmonary inflammation in mice. Inflammation, v. 37, n. 2, p. 566-572, 2014. Available from: https://doi.org/10.1007/s10753-013-9770-4. DOI: https://doi.org/10.1007/s10753-013-9770-4
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